8PD4
Crystal structure of TRIM58 PRY-SPRY domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-29 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.059, 76.531, 107.226 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.290 - 2.714 |
| R-factor | 0.205 |
| Rwork | 0.202 |
| R-free | 0.26420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.030 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.290 | 2.720 |
| High resolution limit [Å] | 2.710 | 2.710 |
| Number of reflections | 14294 | 139 |
| <I/σ(I)> | 7.5 | 2 |
| Completeness [%] | 96.5 | 100 |
| Redundancy | 6.2 | 6.3 |
| CC(1/2) | 0.995 | 0.885 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 25% w/v PEG8000, 0.2 M LiCl |
