8P9C
Crystal structure of p63-p73 heterotetramer (tetramerisation domain) in complex with darpin 1810 F11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.024, 60.312, 53.576 |
| Unit cell angles | 90.00, 100.26, 90.00 |
Refinement procedure
| Resolution | 48.730 - 1.760 |
| R-factor | 0.17184 |
| Rwork | 0.170 |
| R-free | 0.21012 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.470 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.730 | 1.820 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.049 | 0.392 |
| Rmeas | 0.063 | 0.484 |
| Rpim | 0.031 | 0.241 |
| Number of reflections | 25297 | 2481 |
| <I/σ(I)> | 12.2 | 2.1 |
| Completeness [%] | 96.7 | |
| Redundancy | 4 | 4.1 |
| CC(1/2) | 0.997 | 0.899 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 0.2M ammonium acetate, 25% PEG3350, 0.1M HEPES pH 7.5 |
