8P8E

Crystal structure of endolysin gp46 from Pseudomonas aeruginosa bacteriophage vB_PaeM_KTN6

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97929
Spacegroup name	P 21 21 21
Unit cell lengths	41.796, 43.201, 126.181
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.003 - 1.391
Rwork	0.126
R-free	0.16410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.419
Data reduction software	XDS (Nov 1, 2016)
Data scaling software	Aimless (0.5.31)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.200	1.470
High resolution limit [Å]	1.390	1.390
Rmerge	0.059	1.002
Rpim	0.026	0.425
Number of reflections	46757	6679
<I/σ(I)>	15.8	1.8
Completeness [%]	99.9	99.7
Redundancy	6.4	6.4
CC(1/2)	0.999	0.695

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	10 mM HEPES-NaOH pH 7.0; 10 mM trisodium citrate; 16% (w/v) PEG 6000