8P88
X-ray structure of cardiotoxic light chain H3 in complex to neutralizing nanobody C4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-17 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 73.593, 121.953, 88.675 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.797 - 2.022 |
R-factor | 0.2229 |
Rwork | 0.222 |
R-free | 0.23340 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.605 |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.240 | 2.160 |
High resolution limit [Å] | 2.020 | 2.020 |
Number of reflections | 21041 | 21041 |
<I/σ(I)> | 1.7 | |
Completeness [%] | 100.0 | |
Redundancy | 12.7 | |
CC(1/2) | 0.990 | 0.650 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 295.15 | 0.1 M Sodium citrate, 20% w/v PEG 4000, pH 4.5 |