8P5D

Spraguea lophii ribosome in the closed conformation by cryo sub tomogram averaging

This is a non-PDB format compatible entry.

Replaces:  8BR3

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	FEI TITAN KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Detector	GATAN K2 SUMMIT (4k x 4k),GATAN K3 BIOQUANTUM (6k x 4k)

3D Reconstruction

Method	SUBTOMOGRAM AVERAGING
Resolution (Å)	10.8
Software	IMOD,RELION,Warp,SerialEM
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION