8P3J

Fusion hSlp2-a_Rab27A non-covalent complex with hit fragment IMP-1704

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-02
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	62.436, 75.828, 118.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.880 - 2.160
R-factor	0.2875
Rwork	0.284
R-free	0.35080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.173
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.17_3644: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.880	2.237
High resolution limit [Å]	2.160	2.160
Number of reflections	30872	3056
<I/σ(I)>	2.8
Completeness [%]	96.4
Redundancy	1.9
CC(1/2)	0.972	0.325

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.15 M NH4SO4, 0.1 M MES, pH 6.0, 15% PEG 4K