8P2M
C. elegans TIR-1 protein.
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | GIF Quantum LS |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.82 |
| Number of Particle | 370021 |
| Software | cryoSPARC,EPU,REFMAC,UCSF ChimeraX,ISOLDE,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
