8P1Q

USP28 in complex with FT206

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-09
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.873
Spacegroup name	P 21 2 21
Unit cell lengths	100.444, 105.541, 178.708
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.090 - 2.790
R-factor	0.2132
Rwork	0.212
R-free	0.23550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.527
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.090	3.042
High resolution limit [Å]	2.756	2.756
Number of reflections	34993	2917
<I/σ(I)>	12.8
Completeness [%]	94.6
Redundancy	11.9
CC(1/2)	0.998	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.5 M Na-malonate, pH6.0 0.1M Na-citrate, pH 5.0