8P1P

USP28 in complex with AZ1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 2 21
Unit cell lengths	100.444, 105.541, 178.708
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.350 - 2.760
R-factor	0.2138
Rwork	0.213
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.558
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.350	3.008
High resolution limit [Å]	2.760	2.760
Number of reflections	34461	1723
<I/σ(I)>	10.9
Completeness [%]	94.1
Redundancy	13.3
CC(1/2)	0.998	0.393

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.5 M sodium malonate pH 6.0 0.1 M sodium citrate pH 5.0