8P0Z
AP01-S2.3 - a variant of a redesigned transferrin receptor apical domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-12-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.990, 57.370, 39.100 |
Unit cell angles | 90.00, 113.15, 90.00 |
Refinement procedure
Resolution | 35.950 - 1.880 |
R-factor | 0.2143 |
Rwork | 0.213 |
R-free | 0.23630 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.647 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18rc1_3777) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.950 | 1.947 |
High resolution limit [Å] | 1.880 | 1.880 |
Rmerge | 0.192 | 1.189 |
Rmeas | 0.219 | 1.356 |
Rpim | 0.104 | 0.639 |
Number of reflections | 10690 | 1053 |
<I/σ(I)> | 5.72 | 0.75 |
Completeness [%] | 97.1 | 93.5 |
Redundancy | 4.2 | |
CC(1/2) | 0.988 | 0.522 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 11 mg/mL protein in 20 mM HEPES, pH 6.8, 150 mM NaCl mixed with 100 mM MIB buffer, pH 7.5, and 24 % PEG in a ratio 1:2 |