8OOG
Crystal structure of human MAT2a with S-Adenosylmethionine and a fragment bound in a novel pocket
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-30 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 67.880, 94.220, 116.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.370 - 1.384 |
| R-factor | 0.1905 |
| Rwork | 0.190 |
| R-free | 0.20010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.000 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.11.8 (24-FEB-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.374 | 1.408 |
| High resolution limit [Å] | 1.384 | 1.384 |
| Rmerge | 0.070 | 1.097 |
| Rmeas | 0.077 | 1.194 |
| Rpim | 0.031 | 0.466 |
| Total number of observations | 466961 | 23485 |
| Number of reflections | 75517 | 3671 |
| <I/σ(I)> | 13.3 | 2.1 |
| Completeness [%] | 98.7 | |
| Redundancy | 6.2 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 8 % PEG8000, 12 % ethylene glycol, 0.1 M Hepes pH 8.0 |
