8OOD

Crystal structure of human DCAF1 WD40 repeats (Q1250L) in complex with compound 15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.000
Spacegroup name	P 61 2 2
Unit cell lengths	81.496, 81.496, 232.723
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.259 - 1.497
Rwork	0.170
R-free	0.18600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	2.500
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.259	1.520
High resolution limit [Å]	1.497	1.500
Rmerge	0.091	1.726
Rmeas	0.097	1.852
Rpim	0.033	0.652
Total number of observations		26759
Number of reflections	74179	3405
<I/σ(I)>	11.1	1.1
Completeness [%]	99.6
Redundancy	8.7	7.9
CC(1/2)	0.997	0.452

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	2.13M LiSO4, 0.1M Tris pH 7.5