8ONK
Human insulin in complex with the analytical antibody S1 Fab and the analytical antibody HUI-001 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-11 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 278.900, 52.326, 179.504 |
Unit cell angles | 90.00, 101.14, 90.00 |
Refinement procedure
Resolution | 45.610 - 3.403 |
R-factor | 0.2602 |
Rwork | 0.258 |
R-free | 0.31090 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.414 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.610 | 3.525 |
High resolution limit [Å] | 3.403 | 3.403 |
Rmerge | 0.202 | 1.041 |
Rmeas | 0.241 | 1.245 |
Rpim | 0.129 | 0.675 |
Number of reflections | 35523 | 3330 |
<I/σ(I)> | 5.9 | 1.21 |
Completeness [%] | 99.0 | 93.41 |
Redundancy | 3.3 | 3.2 |
CC(1/2) | 0.992 | 0.594 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M TRIS pH 7.5, 15%(w/v) PEG 6000 |