8ONH
Variant Surface Glycoprotein VSG11wt-Oil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-19 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 131.880, 210.771, 133.754 |
| Unit cell angles | 90.00, 104.54, 90.00 |
Refinement procedure
| Resolution | 48.810 - 2.590 |
| R-factor | 0.2312 |
| Rwork | 0.229 |
| R-free | 0.26950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.599 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.810 | 2.683 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Number of reflections | 217669 | 20848 |
| <I/σ(I)> | 14.56 | |
| Completeness [%] | 99.4 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.999 | 0.545 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 19 % (w/v) PEG2000MME 0.2 M NaCl 0.1 M MES/NaOH |
