8OMA
MutSbeta bound to 61bp homoduplex DNA
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | TFS GLACIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 200 |
Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.29 |
Software | cryoSPARC,EPU,REFMAC |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |