8OIO
Crystal structure of the kelch domain of human KLHL12 in complex with PLEKHA4 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-04 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.979499 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.469, 73.086, 103.734 |
Unit cell angles | 90.00, 98.81, 90.00 |
Refinement procedure
Resolution | 79.520 - 1.954 |
R-factor | 0.2021 |
Rwork | 0.200 |
R-free | 0.24860 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.871 |
Data reduction software | XDS (Feb 5, 2021 (BUILT 20210323)) |
Data scaling software | Aimless (0.7.7) |
Phasing software | PHASER (1.20.1_4487) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 79.520 | 1.988 |
High resolution limit [Å] | 1.954 | 1.954 |
Number of reflections | 86247 | 4299 |
<I/σ(I)> | 7.3 | 0.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7 | 6.7 |
CC(1/2) | 0.996 | 0.309 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293.15 | 0.1 M citrate pH 5.5, 0.4 M NaCl, 27,1% PEG8K |