8OIJ
Drosophila Smaug-Smoothened complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-03 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.976254 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 76.590, 76.590, 268.470 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 64.390 - 2.000 |
R-factor | 0.2737 |
Rwork | 0.272 |
R-free | 0.29250 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.528 |
Data reduction software | XDS |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.330 | 2.230 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 21810 | 1090 |
<I/σ(I)> | 7 | |
Completeness [%] | 94.0 | |
Redundancy | 9.9 | |
CC(1/2) | 0.996 | 0.700 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.1M HEPES pH 7.5, 5 % (w/v) PEG 3000, 20 % (w/v) PEG 400 |