8OHT
Crystal structure of Beta-glucuronidase from Acidobacterium capsulatum in complex with competitive inhibitor derrived from siastatin B
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.911880 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 82.750, 44.670, 136.268 |
| Unit cell angles | 90.00, 97.46, 90.00 |
Refinement procedure
| Resolution | 67.648 - 1.050 |
| Rwork | 0.167 |
| R-free | 0.17740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.049 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.650 | 1.070 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Number of reflections | 221410 | 10057 |
| <I/σ(I)> | 15.1 | 1.4 |
| Completeness [%] | 96.5 | |
| Redundancy | 6.3 | |
| CC(1/2) | 0.999 | 0.582 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.5 M AmSO4 1 M LiSO4 0.1 M Trisodium Citrate |
