8OHM

CRYSTAL STRUCTURE OF RNA HELICASE FROM GENOTYPE 1B HEPATITIS C VIRUS: MECHANISM OF UNWINDING DUPLEX RNA

Experimental procedure

Source type	ROTATING ANODE
Source details	MACSCIENCE M18X
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1997-12
Detector	MAC Science DIP-2020
Spacegroup name	P 31 2 1
Unit cell lengths	93.300, 93.300, 104.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.233
Rwork	0.233
R-free	0.30300
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.011 *
RMSD bond angle	1.649 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.030	0.030
Number of reflections	23007
<I/σ(I)>	18	14
Completeness [%]	98.0	92
Redundancy	2.5	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	5.6	4 *	pH 5.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG4000	30 (%)
2	1	1	sodium cacodylate	0.1 (M)
3	1	1	ammonium acetate	0.2 (M)