8OH6

Crystal structure of the Candida albicans 80S ribosome in complex with Paromomycin (500umol)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	173
Detector technology	PIXEL
Collection date	2022-04-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	296.890, 293.380, 443.000
Unit cell angles	90.00, 99.39, 90.00

Refinement procedure

Resolution	175.260 - 3.350
R-factor	0.2844
Rwork	0.284
R-free	0.32760
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.768
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.19rc4_4035: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	175.300	175.300	3.150
High resolution limit [Å]	3.100	50.000	3.100
Rmerge	1.155	0.056	2.728
Rmeas	1.197	0.058	2.825
Rpim	0.312
Number of reflections	1341596	275	63014
<I/σ(I)>	2.64
Completeness [%]	99.5
Redundancy	14.5
CC(1/2)	0.981	0.999	0.030

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277.15	PEG 20K, KSCN, Mg Acetate, K - acetate, Bis-Tris-Acetate, Glycerol, Spermidine, phyllanthoside