8OGB

Crystal structure of human DCAF1 WD40 repeats (Q1250L) in complex with compound 8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-18
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	1.000
Spacegroup name	P 61 2 2
Unit cell lengths	85.018, 85.018, 219.360
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	61.206 - 2.270
Rwork	0.168
R-free	0.21250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	2.049
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	219.360	2.340
High resolution limit [Å]	2.270	2.270
Number of reflections	22640	2016
<I/σ(I)>	14.8
Completeness [%]	100.0
Redundancy	18.8
CC(1/2)	0.998	0.784

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1M Tris pH 7.5, 2.13M Lithium acetate