8OG1

Exostosin-like 3 apo enzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.6199
Spacegroup name	P 31 2 1
Unit cell lengths	120.900, 120.900, 127.450
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.760 - 1.580
R-factor	0.1853
Rwork	0.184
R-free	0.21110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.104
Data reduction software	xia2 (3.dev.661-g1a4ae04e6)
Data scaling software	xia2 (3.dev.661-g1a4ae04e6)
Phasing software	PHENIX (1.18rc1_3769)
Refinement software	PHENIX (1.18rc1_3769)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.900	1.620
High resolution limit [Å]	1.580	1.580
Rmerge	0.079	1.889
Number of reflections	146847	10580
<I/σ(I)>	14.6
Completeness [%]	99.9
Redundancy	11.1
CC(1/2)	0.999	0.323

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	12.5% (w/v) PEG3350, 0.1 M sodium malonate, pH 7.0