8OF4

Nucleosome Bound human SIRT6 (Composite)

EM specimen

Vitrification

Yes

EM Data Collection

Microscope	TFS KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7,0.01
Energy Filter	TFS Selectris X,GIF Quantum LS
Detector	FEI FALCON IV (4k x 4k),GATAN K3 (6k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.94
Number of Particle	2033169
Software	crYOLO,cryoSPARC,RELION,PHENIX,ISOLDE,SerialEM,EPU,UCSF Chimera
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION