8ODX
Interleukin 12 receptor subunit beta-1 Fn domains in complex with antagonistic FAb4 fragment and VHH.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.915 |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 231.402, 231.402, 231.402 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 81.810 - 4.400 |
R-factor | 0.2827 |
Rwork | 0.280 |
R-free | 0.31150 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.824 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 231.400 | 5.910 | 4.670 |
High resolution limit [Å] | 4.400 | 5.390 | 4.400 |
Rmeas | 0.231 | 1.077 | 3.941 |
Number of reflections | 13975 | 1790 | 2193 |
<I/σ(I)> | 18.54 | 6.4 | 1.36 |
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 70 | 71.21 | 71.36 |
CC(1/2) | 1.000 | 0.959 | 0.568 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.34 | 287 | 11.82% Tacsimate,10% Ethylene glycol,10 % PEG smear broad |