8ODP
Structure of human RTCB with GMPCPP in complex with Archease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-05-10 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 98.433, 122.597, 124.759 |
Unit cell angles | 90.00, 107.88, 90.00 |
Refinement procedure
Resolution | 51.290 - 2.300 |
R-factor | 0.1825 |
Rwork | 0.181 |
R-free | 0.21580 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.674 |
Data reduction software | XDS (20210205) |
Data scaling software | Aimless (1.12.10) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 122.600 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.095 | 1.161 |
Rpim | 0.039 | 0.465 |
Number of reflections | 124773 | 6124 |
<I/σ(I)> | 11.4 | 1.5 |
Completeness [%] | 99.8 | |
Redundancy | 7 | |
CC(1/2) | 0.998 | 0.786 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 0.2 M potassium nitrate, 20% w/v PEG 3350 |