8KIB
NADP+-dependent cytosolic isocitrate dehydrogenase complexed with oxaloacetate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-03-07 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 98.512, 93.421, 111.108 |
Unit cell angles | 90.00, 113.85, 90.00 |
Refinement procedure
Resolution | 29.550 - 1.700 |
R-factor | 0.1751 |
Rwork | 0.175 |
R-free | 0.20340 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.875 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.550 | 1.770 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 202031 | 20124 |
<I/σ(I)> | 28.5 | |
Completeness [%] | 99.9 | |
Redundancy | 4.1 | |
CC(1/2) | 0.996 | 0.830 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | PEG 4000, sodium citrate, magnesium chloride, oxaloacetate |