8KHV
The crystal structure of glycosaminoglycan lyase GAGase II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-06-14 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.917, 82.096, 79.885 |
Unit cell angles | 90.00, 106.59, 90.00 |
Refinement procedure
Resolution | 44.010 - 1.900 |
R-factor | 0.1602 |
Rwork | 0.159 |
R-free | 0.19240 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AutoSol |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.010 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.092 | 0.206 |
Rmeas | 0.100 | 0.230 |
Rpim | 0.038 | 0.100 |
Number of reflections | 86421 | 4092 |
<I/σ(I)> | 12.9 | 6.1 |
Completeness [%] | 98.5 | 92.04 |
Redundancy | 6.7 | 4.9 |
CC(1/2) | 0.992 | 0.964 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M HEPES (pH 7.0), 12% (w/v) polyethylene glycol (PEG) 6000 and 0.2 M CaCl2 |