8KH9

Crystal structure of FGFR4(V550M) kinase domain with 8z

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	277
Detector technology	CCD
Collection date	2023-04-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	42.398, 61.605, 60.605
Unit cell angles	90.00, 97.93, 90.00

Refinement procedure

Resolution	36.880 - 1.420
R-factor	0.1709
Rwork	0.170
R-free	0.19260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wcx
RMSD bond length	0.009
RMSD bond angle	1.083
Data reduction software	HKL-3000
Data scaling software	DIALS
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.600	1.460
High resolution limit [Å]	1.420	1.420
Number of reflections	58347	4290
<I/σ(I)>	10.3
Completeness [%]	100.0
Redundancy	6
CC(1/2)	0.996	0.194

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M Bis-Tris (pH 4.5), 0.2 M Li2SO4, 16-19 % PEG 3350