8KH7
Crystal structure of FGFR4 kinase domain with 8zc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2023-04-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.152, 61.237, 60.486 |
| Unit cell angles | 90.00, 98.06, 90.00 |
Refinement procedure
| Resolution | 27.260 - 1.520 |
| R-factor | 0.1659 |
| Rwork | 0.164 |
| R-free | 0.19480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7ybo |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.427 |
| Data reduction software | HKL-3000 |
| Data scaling software | DIALS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.240 | 1.560 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Number of reflections | 46948 | 3481 |
| <I/σ(I)> | 7.5 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.2 | |
| CC(1/2) | 0.984 | 0.361 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M Bis-Tris (pH 4.5), 0.2 M Li2SO4, 16-19% PEG 3350 |
