8KH2

Crystal structure of horse-spleen L-ferritin fused with amyloid beta peptide (1-42).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	93.1
Detector technology	PIXEL
Collection date	2023-08-09
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.542
Spacegroup name	F 4 3 2
Unit cell lengths	181.935, 181.935, 181.935
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.691 - 2.000
Rwork	0.215
R-free	0.25810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.938
Data reduction software	CrysalisPro (v42)
Data scaling software	Aimless (0.7.9)
Phasing software	MOLREP (11.9.02)
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.691	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	17564	1264
<I/σ(I)>	19.8
Completeness [%]	97.7
Redundancy	7.4
CC(1/2)	0.996	0.977

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293.15	Ammonium sulfate (0.5-1.0M), Cadmium sulfate (12-20 mM)