8KE7

Crystal structure of DNA binding and cleavage core of human topoisomerase 2-beta in a DNA binding-competent conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-17
Detector	RAYONIX MX300HE
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	100.719, 113.708, 209.267
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.830 - 2.800
R-factor	0.1952
Rwork	0.194
R-free	0.23810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.811
Data reduction software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.830	2.940
High resolution limit [Å]	2.800	2.840
Rmeas	0.141
Rpim	0.064
Number of reflections	58573	5768
<I/σ(I)>	11.69
Completeness [%]	99.6
Redundancy	4.8
CC(1/2)		0.756

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	ammonium acetate, calcium chloride dehydrate, sodium cacodylate trihydrate, PEG 4000