8K4Z

Crystal structure of human MMP-7 in complex with inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00003
Spacegroup name	I 4
Unit cell lengths	75.799, 75.799, 60.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.598 - 1.700
Rwork	0.203
R-free	0.23920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.555
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.600	1.790
High resolution limit [Å]	1.700	1.700
Number of reflections	18920	2753
<I/σ(I)>	7.9
Completeness [%]	99.9
Redundancy	11.9
CC(1/2)	0.992	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	20% w/v Polyethylene glycol 3350, 100mM Bis-Tris propane pH7.5, 200mM Sodium iodide