8K3L
SOD1 and Nanobody3 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.214, 62.442, 314.928 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.070 - 2.300 |
R-factor | 0.1971 |
Rwork | 0.195 |
R-free | 0.23470 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.949 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 6.240 | 2.300 |
Rmerge | 0.120 | 0.047 | 1.101 |
Rmeas | 0.125 | 0.049 | 1.144 |
Rpim | 0.034 | 0.014 | 0.308 |
Total number of observations | 739399 | ||
Number of reflections | 55961 | 3051 | 2732 |
<I/σ(I)> | 3 | ||
Completeness [%] | 99.7 | 99.3 | 100 |
Redundancy | 13.2 | 11.6 | 13.5 |
CC(1/2) | 0.996 | 0.999 | 0.908 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.2 M Ammonium acetate, 0.1 M Sodium acetate trihydrate pH 4.6, 30% w/v Polyethylene, glycol monomethyl ether 2,000 |