8JWJ
PHD Finger Protein 7 (PHF7) in complex with UBE2D2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-04-14 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97403 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 102.304, 106.825, 145.759 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.350 - 2.960 |
R-factor | 0.1959 |
Rwork | 0.194 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1weq 6hpr AlphaFold |
RMSD bond length | 0.007 |
RMSD bond angle | 0.950 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (1.19.2_4158) |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.350 | 3.067 |
High resolution limit [Å] | 2.960 | 2.961 |
Rmerge | 0.067 | |
Rmeas | 0.075 | |
Rpim | 0.034 | |
Number of reflections | 32768 | 3144 |
<I/σ(I)> | 14.5 | |
Completeness [%] | 96.5 | 94.39 |
Redundancy | 4.5 | 4.6 |
CC(1/2) | 0.998 | 0.716 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 277 | MPD, Tris |