8JRC
Crystal structure of the GAF domain of the transcriptional activator NifA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.631, 70.093, 55.039 |
| Unit cell angles | 90.00, 113.06, 90.00 |
Refinement procedure
| Resolution | 45.308 - 2.300 |
| R-factor | 0.2087 |
| Rwork | 0.206 |
| R-free | 0.25850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.578 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.308 | 2.382 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 16693 | 1679 |
| <I/σ(I)> | 13.22 | |
| Completeness [%] | 92.7 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.998 | 0.925 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 310.15 | 22% PEG 3350, 50mM HEPES pH 7.0 |
