8JR3

Crystal structure of Hendra Virus attachment(G) glycoprotein mutant S586N in complex with neutralizing antibody 14F8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-23
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9789
Spacegroup name	C 2 2 21
Unit cell lengths	110.843, 257.014, 193.518
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.790 - 3.220
R-factor	0.2167
Rwork	0.215
R-free	0.24130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.413
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.790	3.290
High resolution limit [Å]	3.220	3.230
Number of reflections	44694	44663
<I/σ(I)>	6.57
Completeness [%]	99.0
Redundancy	5.1
CC(1/2)	0.977	0.662

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	PEG3350 13.5%, 100mM pH 4.7 NaAC