8JQW
Crystal structure of V30I mutated human transthyretin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-24 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 43.170, 85.640, 64.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.550 - 1.800 |
R-factor | 0.1786 |
Rwork | 0.176 |
R-free | 0.22160 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.894 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.280 | 1.890 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.078 | 0.312 |
Number of reflections | 22870 | 3273 |
<I/σ(I)> | 15.6 | 5.5 |
Completeness [%] | 99.9 | |
Redundancy | 6.9 | |
CC(1/2) | 0.997 | 0.946 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | PEG400, MgCl2, glycerol |