8JNR

Crystal structure of human ALKBH3 bound to 3mC containing ssDNA through distal crosslink

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9875
Spacegroup name	P 1 21 1
Unit cell lengths	108.615, 142.171, 130.110
Unit cell angles	90.00, 113.07, 90.00

Refinement procedure

Resolution	34.380 - 3.660
R-factor	0.229
Rwork	0.227
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2UIW
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.720
High resolution limit [Å]	3.660	9.910	3.660
Rmerge	0.244	0.044	0.694
Total number of observations	97662
Number of reflections	35958	1729	1824
<I/σ(I)>	5.2
Completeness [%]	89.9	83.9	92
Redundancy	2.7	2.8	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1M NaCl, 20% PEG6000