8JNR
Crystal structure of human ALKBH3 bound to 3mC containing ssDNA through distal crosslink
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9875 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 108.615, 142.171, 130.110 |
| Unit cell angles | 90.00, 113.07, 90.00 |
Refinement procedure
| Resolution | 34.380 - 3.660 |
| R-factor | 0.229 |
| Rwork | 0.227 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2UIW |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.720 |
| High resolution limit [Å] | 3.660 | 9.910 | 3.660 |
| Rmerge | 0.244 | 0.044 | 0.694 |
| Total number of observations | 97662 | ||
| Number of reflections | 35958 | 1729 | 1824 |
| <I/σ(I)> | 5.2 | ||
| Completeness [%] | 89.9 | 83.9 | 92 |
| Redundancy | 2.7 | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M NaCl, 20% PEG6000 |
