8JKT
Crystal structure of feline aminopeptidase N ectodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-08-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97835 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 123.200, 132.588, 129.364 |
| Unit cell angles | 90.00, 101.18, 90.00 |
Refinement procedure
| Resolution | 35.830 - 1.900 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| R-free | 0.22050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.209 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (v1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.830 | 5.160 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 318333 | 17090 |
| <I/σ(I)> | 18.3 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.996 | 0.996 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2M Sodium Thiocyanate pH 6.9, 20% w/v PEG 3350 |
