8JKT
Crystal structure of feline aminopeptidase N ectodomain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2022-08-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97835 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 123.200, 132.588, 129.364 |
Unit cell angles | 90.00, 101.18, 90.00 |
Refinement procedure
Resolution | 35.830 - 1.900 |
R-factor | 0.178 |
Rwork | 0.178 |
R-free | 0.22050 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.209 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (v1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.830 | 5.160 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 318333 | 17090 |
<I/σ(I)> | 18.3 | |
Completeness [%] | 99.9 | |
Redundancy | 6.7 | |
CC(1/2) | 0.996 | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2M Sodium Thiocyanate pH 6.9, 20% w/v PEG 3350 |