8JKQ
T95R mutant IRF4 DNA-binding domain bound to an DNA containing GACA motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-05-13 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.9752 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.630, 68.890, 247.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.080 - 3.090 |
R-factor | 0.2458 |
Rwork | 0.244 |
R-free | 0.27080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8jkl |
RMSD bond length | 0.003 |
RMSD bond angle | 0.963 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.710 | 3.170 |
High resolution limit [Å] | 3.090 | 3.090 |
Rmerge | 0.182 | 1.270 |
Rmeas | 0.190 | 1.321 |
Rpim | 0.053 | 0.360 |
Total number of observations | 223083 | 17005 |
Number of reflections | 17407 | 1281 |
<I/σ(I)> | 10.8 | 2.6 |
Completeness [%] | 99.2 | |
Redundancy | 12.8 | 13.3 |
CC(1/2) | 0.997 | 0.984 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M sodium citrate (pH 5.0) and 10% (w/v) PEG 6000 |