8JKO
T95R mutant IRF4 DNA-binding domain bound to an DNA containing GATA motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9752 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 116.766, 116.766, 153.008 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.010 - 2.950 |
| R-factor | 0.2318 |
| Rwork | 0.231 |
| R-free | 0.25220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8jkl |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.208 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
| High resolution limit [Å] | 2.950 | 8.000 | 2.950 |
| Rmerge | 0.177 | 0.105 | 2.053 |
| Rmeas | 0.182 | 0.108 | 2.105 |
| Rpim | 0.041 | 0.026 | 0.464 |
| Total number of observations | 522535 | ||
| Number of reflections | 26068 | 1421 | 1288 |
| <I/σ(I)> | 2.9 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 20 | 17.9 | 20.4 |
| CC(1/2) | 0.933 | 0.998 | 0.578 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M MgCl2, 0.1 M sodium citrate (pH 5.0) and 15% (w/v) PEG 4000 |
