Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

8JIB

Crystal Structure of Prophenoloxidase PPO6 from Aedes aegypti

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X17B1
Synchrotron siteNSLS
BeamlineX17B1
Temperature [K]100
Detector technologyPIXEL
Collection date2022-11-19
DetectorDECTRIS EIGER X 9M
Wavelength(s)0.920
Spacegroup nameP 1 21 1
Unit cell lengths121.041, 364.149, 125.522
Unit cell angles90.00, 118.95, 90.00
Refinement procedure
Resolution48.660 - 3.150
R-factor0.27134
Rwork0.269
R-free0.31041
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.005
RMSD bond angle1.286
Data reduction softwareXDS
Data scaling softwareAimless
Phasing softwareMR-Rosetta
Refinement softwareREFMAC (5.8.0419)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]48.6603.320
High resolution limit [Å]3.1503.150
Rmerge0.2410.910
Rmeas0.278
Rpim0.1350.499
Number of reflections16174623632
<I/σ(I)>51.5
Completeness [%]98.898.8
Redundancy3.83.7
CC(1/2)0.9670.429
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.5291.150.2 M MgCl2,0.1 M Sodium Cacodylate pH6.5,10%-15% v/v PEG 1000

226707

PDB entries from 2024-10-30

PDB statisticsPDBj update infoContact PDBjnumon