8J0R

Structure of human TFAP2A in complex with DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 32 2 1
Unit cell lengths	124.790, 124.790, 96.917
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.990 - 2.100
R-factor	0.1638
Rwork	0.162
R-free	0.19411
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.789
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	108.100	2.160
High resolution limit [Å]	2.100	2.100
Number of reflections	51127	4160
<I/σ(I)>	15
Completeness [%]	100.0
Redundancy	9.1
CC(1/2)	0.998	0.825

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M citric acid (pH3.5), 34% PEG 200 (w/v).