8IZU
Crystal structure of the N-terminal domain (residues 1-137) of MPXV A7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-12-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97851 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 37.903, 140.327, 153.519 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.450 - 2.540 |
R-factor | 0.20288 |
Rwork | 0.201 |
R-free | 0.23776 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8izt |
RMSD bond length | 0.008 |
RMSD bond angle | 1.578 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.450 | 2.650 |
High resolution limit [Å] | 2.540 | 2.540 |
Number of reflections | 14013 | 1676 |
<I/σ(I)> | 14.1 | |
Completeness [%] | 99.8 | |
Redundancy | 12.8 | |
CC(1/2) | 0.997 | 0.954 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Potassium thiocyanate, 30% w/v Polyethylene glycol monomethyl ether 2000 |