8IZT
Crystal structure of the N-terminal domain (residues 1-121) of MPXV A7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 75.950, 31.263, 49.838 |
| Unit cell angles | 90.00, 112.34, 90.00 |
Refinement procedure
| Resolution | 35.100 - 1.740 |
| Rwork | 0.172 |
| R-free | 0.20570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6cb6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.539 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.100 | 1.770 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Number of reflections | 10782 | 612 |
| <I/σ(I)> | 15 | |
| Completeness [%] | 99.7 | |
| Redundancy | 6.2 | |
| CC(1/2) | 0.999 | 0.255 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0,01 M Zinc chloride, 0.1 M MES pH 6.0, 20% w/v PEG6000 |
