8IQL

Structural basis of the specificity and interaction mechanism of Bmf binding to pro-survival proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-30
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 32 2 1
Unit cell lengths	122.743, 122.743, 86.431
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.787 - 2.958
R-factor	0.1925
Rwork	0.189
R-free	0.22620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.570
Data reduction software	xia2
Data scaling software	SCALEPACK
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.787	3.063
High resolution limit [Å]	2.958	2.958
Number of reflections	30365	1605
<I/σ(I)>	12.9	2.29
Completeness [%]	99.9
Redundancy	10.7
CC(1/2)	0.997	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M potassium thiocyanate, polyethylene glycol monomethyl ether 2,000