8IKV

pUbl depleted phospho-Parkin(K211N,R163D) in complex with pUb

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-22
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 1 21 1
Unit cell lengths	45.450, 76.426, 114.329
Unit cell angles	90.00, 100.48, 90.00

Refinement procedure

Resolution	38.242 - 2.350
Rwork	0.198
R-free	0.21690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5caw
RMSD bond length	0.002
RMSD bond angle	0.836
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASES
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.390	2.430
High resolution limit [Å]	2.350	2.350
Number of reflections	31088	3040
<I/σ(I)>	7.7
Completeness [%]	96.5
Redundancy	3.1
CC(1/2)	0.995	0.686

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.15 M Potassium bromide, 30% w/v Polyethylene glycol monomethyl ether 2,000