8IJ9

Crystal structure of the ELKS1/Rab6B complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	49.193, 93.227, 53.077
Unit cell angles	90.00, 103.74, 90.00

Refinement procedure

Resolution	51.560 - 2.040
R-factor	0.1745
Rwork	0.172
R-free	0.22510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.129
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (v1.19.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.560	2.150
High resolution limit [Å]	2.040	2.040
Rmerge	0.076	0.294
Rmeas	0.098	0.390
Rpim	0.060	0.254
Total number of observations	59879	4710
Number of reflections	26796	2644
<I/σ(I)>	7.2	2.4
Completeness [%]	90.2
Redundancy	2.2	1.8
CC(1/2)	0.989	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	0.1 M HEPES pH 7.5 and 42 % v/v Polyethylene glycol 200