8IJ9
Crystal structure of the ELKS1/Rab6B complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.193, 93.227, 53.077 |
| Unit cell angles | 90.00, 103.74, 90.00 |
Refinement procedure
| Resolution | 51.560 - 2.040 |
| R-factor | 0.1745 |
| Rwork | 0.172 |
| R-free | 0.22510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.129 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.19.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.560 | 2.150 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.076 | 0.294 |
| Rmeas | 0.098 | 0.390 |
| Rpim | 0.060 | 0.254 |
| Total number of observations | 59879 | 4710 |
| Number of reflections | 26796 | 2644 |
| <I/σ(I)> | 7.2 | 2.4 |
| Completeness [%] | 90.2 | |
| Redundancy | 2.2 | 1.8 |
| CC(1/2) | 0.989 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M HEPES pH 7.5 and 42 % v/v Polyethylene glycol 200 |
