8IDI

Crystal structure of nanobody VHH-T71 with MERS-CoV RBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	238.549, 46.835, 95.946
Unit cell angles	90.00, 112.52, 90.00

Refinement procedure

Resolution	36.551 - 1.901
R-factor	0.1747
Rwork	0.173
R-free	0.20710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.551	1.969
High resolution limit [Å]	1.901	1.901
Number of reflections	77670	7647
<I/σ(I)>	21.16
Completeness [%]	99.8
Redundancy	6.4
CC(1/2)	0.995	0.869

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	4% v/v Tacsimate, pH 6.0, 12% w/v PEG 3350