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8IDF

Crystal structure of human TUT1 complexed with U6 snRNA

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsPHOTON FACTORY BEAMLINE BL-17A
Synchrotron sitePhoton Factory
BeamlineBL-17A
Temperature [K]100
Detector technologyPIXEL
Collection date2021-11-03
DetectorDECTRIS EIGER X 16M
Wavelength(s)1.27
Spacegroup nameP 65 2 2
Unit cell lengths78.724, 78.724, 370.272
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution19.990 - 3.700
R-factor0.3063
Rwork0.305
R-free0.33860
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)5wu1
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwarePHASER
Refinement softwarePHENIX (1.18.2_3874)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]19.99019.9903.790
High resolution limit [Å]3.70016.5303.700
Rmerge0.5320.0994.401
Rmeas0.5380.1014.453
Total number of observations576696
Number of reflections13674150975
<I/σ(I)>8.9935.911.48
Completeness [%]99.495.593
Redundancy42.17536.0442.821
CC(1/2)0.9970.9970.753
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP6277100mM BisTris pH 6.4, 18% PEG 3350, 2% Tacsimate pH 6.0, 8% Acetonitrile

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