8IDF
Crystal structure of human TUT1 complexed with U6 snRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.27 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 78.724, 78.724, 370.272 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.990 - 3.700 |
R-factor | 0.3063 |
Rwork | 0.305 |
R-free | 0.33860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5wu1 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.990 | 19.990 | 3.790 |
High resolution limit [Å] | 3.700 | 16.530 | 3.700 |
Rmerge | 0.532 | 0.099 | 4.401 |
Rmeas | 0.538 | 0.101 | 4.453 |
Total number of observations | 576696 | ||
Number of reflections | 13674 | 150 | 975 |
<I/σ(I)> | 8.99 | 35.91 | 1.48 |
Completeness [%] | 99.4 | 95.5 | 93 |
Redundancy | 42.175 | 36.04 | 42.821 |
CC(1/2) | 0.997 | 0.997 | 0.753 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 100mM BisTris pH 6.4, 18% PEG 3350, 2% Tacsimate pH 6.0, 8% Acetonitrile |